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KMID : 0894520220260020079
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Development & Reproduction
2022 Volume.26 No. 2 p.79 ~ p.90
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Zebrafish Klf11b is Required to Maintain Cell Viability by Inhibiting p53-Mediated Apoptosis
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Kong Hee-Jeong
Lee Jung-Jin
Kim Ju-Won
Kim Ju-Lan
Kim Young-Ok
Yeo Sang-Yeob
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Abstract
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Kruppel-like factor 10 (KLF10) regulates various cellular functions, such as proliferation, differentiation and apoptosis, as well as the homeostasis of several types of tissue. In the present study, we attempted a loss-of-function analysis of zebrafish Klf11a and Klf11b, which constitute human KLF10 homologs. Embryos injected with klf11b-morpholino (MO) showed developmental retardation and cell death, whereas klf11a-MO-injected embryos showed normal development. In klf11b-MO-injected embryos, a dramatic increase in the amount of zebrafish p53 mRNA might be the cause of the increase in that of bax. The degree of apoptosis decreased in the klf11b-MO and p53-MO co-injected embryos. These findings imply that KLF10 is a negative regulator of p53-dependent transcription, suggesting that the KLF10/p53 complex may play an important role in apoptosis for maintenance of tissue homeostasis during embryonic development.
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KEYWORD
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Klf11b, Kruppel-like factor 10 (KLF10), p53, Zebrafish
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